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DNA methylation is the best-studied epigenetic modification and altered DNA methylation patterns have been identified in cancer and many other complex diseases.The first DNA methylation-based biomarkers for early diagnosis of cancer have been approved by regulatory agencies, which illustrate the potential and advantages of DNA methylation analysis.
The choice of the best-suited assay depends on the biological question to be investigated, the quantity and quality of biological material available, and access to specific instrumentation.