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Pyrosequencing is a replication-based sequencing method in which addition of the correct nucleotide to immobilized template DNA is signaled by a photometrically detectable reaction.The fundamental basis of pyrosequencing is that pyrophosphate is released when a deoxyribonucleotide triphosphate is added to the end of a nascent strand of DNA.
Pyrosequencing is used to reveal the genetic code of a section of DNA. It is also able to detect single nucleotide polymorphisms, insertion-deletions or other sequence variations, in addition to being able to quantify DNA methylation and allele frequency.